Acta Chimica Slovaca (ACS) publishes papers on fundamental and applied aspects of chemistry, biochemistry, chemical technology, chemical engineering and process control, biotechnology and food technology. Welcome are also topics which include chemical aspects of materials, physical chemistry and chemical physics, analytical chemistry, macromolecular chemistry and biomedical engineering.

Influence of pH on adsorption of human immunoglobulin gamma, human serum albumin and horse myoglobin by commercial chromatographic materials designed for downstream processing of monoclonal antibodies

Michal Gramblička, Darina Tóthová, Monika Antošová, Milan Polakovič *

Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovakia

E-mail: * milan.polakovic@stuba.sk

Abstract: Static adsorption capacity was measured for three different proteins: human immunoglobulin gamma, human serum albumin and horse skeletal muscle myoglobin. Eight commercial chromatographic materials were tested: FractoGel EMD SE HiCap (M), SP Sepharose Fast Flow, S Ceramic HyperD F, MabSelect, rProtein A Sepharose Fast Flow, Poros 50A High Capacity, ProSep –vA High Capacity and MEP HyperCel. The adsorption capacity was measured at pH values of 4.0, 5.0, 6.0, 7.0 and 8.0.

Keywords: adsorption capacity, chromatography, horse myoglobin, human immunoglobulin gamma, human serum albumin

Full paper in Portable Document Format: acs_0008.pdf

Acta Chimica Slovaca, Vol. 1, No. 1, 2008, pp. 85—94