Acta Chimica Slovaca (ACS) publishes papers on fundamental and applied aspects of chemistry, biochemistry, chemical technology, chemical engineering and process control, biotechnology and food technology. Welcome are also topics which include chemical aspects of materials, physical chemistry and chemical physics, analytical chemistry, macromolecular chemistry and biomedical engineering.

Effect of new synthetically prepared quinolone ethyl-1,4-dihydro-8-nitro-4-oxoquinoline-3-carboxylate on human leukemia cell line HL-60 without/with presence of UVA irradiation

Soňa Jantová *, Silvia Letašiová a, Katarína Konariková b, Viktor Milata c, Vlasta Brezová d

Institute of Biochemistry, Nutrition and Health Protection, Faculty of Chemical and Food Technology, STU, Radlinského 9, SK-81237 Bratislava
a MatTek In Vitro Life Science Laboratories, Sládkovičova 2, SK-811 06 Bratislava
b Institute of Organic Chemistry, Catalysis and Petrochemistry Faculty of Chemical and Food Technology, STU, Radlinského 9, SK-81237 Bratislava
c Institute of Organic Chemistry, Catalysis and Petrochemistry Faculty of Chemical and Food Technology, STU, Radlinského 9, SK-81237 Bratislava
d Institute of Physical Chemistry and Chemical Physics, Faculty of Chemical and Food Technology, STU, Radlinského 9, SK-81237 Bratislava

E-mail: * sona.jantova@stuba.sk

Abstract: The present study demonstrated cytotoxic/phototoxic effects of new synthetically prepared quinolone ethyl-1,4-dihydro-8-nitro-4-oxoquinoline-3-carboxylate (DNQC) on human leukemia cells HL-60. The effects on cell proliferation, cell cycle, induction of apoptotic/necrotic cell death and DNA damage in dark and in the presence of UVA irradiation were evaluated. DNQC induced a different cytotoxic/phototoxic effect which was concentration- and time-dependent. UVA irradiation statistically increased DNQC effect on HL-60 cells. The IC50 and IC100 values showed that the irradiated HL-60 cells are 5.4 – 27.0 (IC50) and 2.5 – 15.0 (IC100) times more sensitive in comparison to the non-irradiated cells. The cytotoxic/phototoxic concentrations of DNQC induced necrotic death of leukemia cells HL-60. Dead cells had the integrity damage of cytoplasmic membrane and DNA damage. The DNA damage generated by DNQC alone/with combination of UVA irradiation induced cell cycle changes and led to necrosis. The concomitant effect of necrosis was the release of lactate dehydrogenase (LDH) from HL-60 cells with integrity damage of cytoplasmic membrane.

Keywords: ethyl-1,4-dihydro-8-nitro-4-oxoquinoline-3-carboxylate; leukemia cells HL-60, UVA irradiation, Cytotoxicity/Phototoxicity; cell death, DNA damage

Full paper in Portable Document Format: acs_0058.pdf

Acta Chimica Slovaca, Vol. 3, No. 1, 2010, pp. 51—72