Photodynamic inactivation of Escherichia coli by methylene blue incorporated in ZSM-5 zeolite channels under red LED light
Miroslava Smolinská *, Petra Olejníková a, Daniela Hudecová b, Gabriel Čík
Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, STU, Radlinského 9, Bratislava 812 37, Slovak Republic
a Institute of Biochemistry, Nutrition and Health Protection, Faculty of Chemical and Food Technology, STU, Radlinského 9, Bratislava 812 37, Slovak Republic
b Institute of Biochemistry, Nutrition and Health Protection, Faculty of Chemical and Food Technology, STU, Radlinského 9, Bratislava 812 37, Slovak Republic
E-mail: * miroslava.smolinska@stuba.sk
Abstract: Photodynamic therapy (PDT) is a method that combines the use of nontoxic components, namely photosensitizer, light and oxygen to cause localised oxidative photo-induced damage. The aim of this study was to contribute to PDT development by studying an alternative light sources using red light-emitting diode (LED) light and evaluated its effect on the photodynamic activity of methylene blue (MB) in biological experiments. To prepare a new heterogeneous photocatalyst by incorporating the MB into ZSM-5 zeolite structures, which effectively produces ROS by photoactivation with visible light. The investigations of the effectiveness of PDT with red LED light and MB, afterwards with ZSM-5-MB were carried out on Gram-negative bacteria Escherichia coli CCM 3988.
Keywords: methylene blue, zeolite, Escherichia coli, light-emitting diode, inhibition
Full paper in Portable Document Format: acs_0057.pdf
Acta Chimica Slovaca, Vol. 3, No. 1, 2010, pp. 41—50