Acta Chimica Slovaca (ACS) publishes papers on fundamental and applied aspects of chemistry, biochemistry, chemical technology, chemical engineering and process control, biotechnology and food technology. Welcome are also topics which include chemical aspects of materials, physical chemistry and chemical physics, analytical chemistry, macromolecular chemistry and biomedical engineering.

Flow-through electrochemical system with the DNA-based biosensor for the evaluation of deep DNA damage by chemicals and effect of antioxidants

Darina Šimková *, Ernest Beinrohr, Ján Labuda †

Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 81237 Bratislava, Slovakia

E-mail: * simkovad@gmail.com, † jan.labuda@stuba.sk

Abstract: In this work we present an electrochemical DNA biosensor arranged in a flow-through cell which was used for the evaluation of DNA damage. The DNA-based biosensor is created using a layer double stranded calf thymus DNA deposited on the surface of a screen-printed carbon electrode. The DNA damage by the Fenton reaction agents is detected by cyclic voltammetry parameters of the [Fe(CN)6]3–/4- complex used as a redox indicator in the phosphate buffer solution. Electrochemical impedance spectroscopy is used for the verification of changes of the DNA layer. The biosensor was also used for the detection of antioxidative effects of standard chemicals and tea extracts.

Keywords: DNA damage, biosensor, flow-through electrochemical system, screen-printed carbon electrode

Full paper in Portable Document Format: acs_0052.pdf

Acta Chimica Slovaca, Vol. 2, No. 2, 2009, pp. 129—138