Acta Chimica Slovaca (ACS) publishes papers on fundamental and applied aspects of chemistry, biochemistry, chemical technology, chemical engineering and process control, biotechnology and food technology. Welcome are also topics which include chemical aspects of materials, physical chemistry and chemical physics, analytical chemistry, macromolecular chemistry and biomedical engineering.

Study on secondary metabolites of Trichoderma atroviride F742 and their role in antibiosis

Tomáš Pagáč *, Zuzana Kubová, Ján Víglaš, Zuzana Kavalová, Petra Olejníková †

Institute of Biochemistry and Microbiology, Faculty of Food and Chemical Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37, Bratislava, Slovakia

E-mail: * tomaspagac1@gmail.com, † petra.olejnikova@stuba.sk

Abstract: Soil-borne pathogenic fungi are widely distributed and are responsible for serious damage within agriculture. The traditional approach to control soil-borne pathogens leads to the usage of chemical fungicides, many of which have proven harmful side effects on the environment. As an increased mycoparasitic activity of mutant strain Trichoderma atroviride F742 has been observed, its use as a biocontrol agent might be considered. Mycoparasitic fungi of the genus Trichoderma have been used for biocontrol of pathogens as well as for their ability to promote plant growth. In this work we focused on mycoparasitic activity of the strain Trichoderma atroviride F742 prepared by UV mutagenesis (from parental strain T. atroviride F534) and the physiological role of T. atroviride metabolites in antibiosis. T. atroviride F742 has remarkable mycoparasitic activity, is able to attack and colonize phytopathogens (Alternaria alternata, Botrytis cinerea, Fusarium culmorum). Isolated metabolites were able to inhibit the growth of bacteria yeasts and fungi Candida albicans, A. alternata and F. culmorum. As different expression pattern following the secondary metabolites production of ABC transporters has been observed, we suggest their role in transport of secondary metabolites produced by T.atroviride F745.

Keywords:

Trichoderma sp., secondary metabolites, mycoparasitism, ABC transporters, antimicrobial potential of produced metabolites

Acta Chimica Slovaca, Vol. 16, No. 1, 2023, pp. 12—21, DOI: 10.2478/acs-2023-0002