Acta Chimica Slovaca (ACS) publishes papers on fundamental and applied aspects of chemistry, biochemistry, chemical technology, chemical engineering and process control, biotechnology and food technology. Welcome are also topics which include chemical aspects of materials, physical chemistry and chemical physics, analytical chemistry, macromolecular chemistry and biomedical engineering.

Characterization of the growth of Lactobacillus plantarum in milk in dependence on temperature

Zuzana Matejčeková *, Denisa Liptáková, Sabína Spodniaková, Ľubomír Valík

Department of Nutrition and Food Quality Assessment, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, SK 812 37, Bratislava

E-mail: * zuzana.matejcekova@stuba.sk

Abstract: The effect of temperature on the growth dynamics of Lactobacillus plantarum in the model environment of UHT milk was investigated. Based on the experiments between temperatures ranged from 8 to 40 °C, growth dynamics of the studied isolate was positively determined by the cultivation temperature that led to increasing intensity of growth in the exponential phase (except for temperatures 38 and 40 °C). Final counts in stationary phase have reached concentrations about 107 CFU·ml–1 from initial 103 CFU·ml–1, except for marginal 8 and 40 °C. Experimentally, it was found, that optimal temperature was close to 37 °C, where the fastest specific growth rate in an exponential phase was recorded (m = 0.7683 h–1, td = 54 min) and the stationary phase was reached after 24 h of incubation. During the growth and multiplication of Lb. plantarum, no significant decrease of pH values in comparison to initial ones in dependence on temperature were determined (0.00—0.24 units).

Keywords: lactic acid bacteria, Lactobacillus plantarum, predictive microbiology

Full paper in Portable Document Format: acs_0249.pdf

Acta Chimica Slovaca, Vol. 9, No. 2, 2016, pp. 104—108, DOI: 10.1515/acs-2016-0018