Identification of biodegradation products of biphenyl and 2,3-dihydroxybiphenyl (2,3-DHB)
Slavomíra Murínová *, Katarína Dercová, Peter Tarábek a, Peter Tölgyessy b
Slovak University of Technology, Faculty of Chemical and Food Technology, Institute of Biotechnology and Food Science, Department of Biochemical Technology, Radlinského 9, 812 37 Bratislava, Slovakia
a Water Research Institute, Nábrežie arm. gen. L. Svobodu 5, 812 49 Bratislava, Slovakia
b Water Research Institute, Nábrežie arm. gen. L. Svobodu 5, 812 49 Bratislava, Slovakia
E-mail: * slavomira.murinova@gmail.com
Abstract: We investigated the degradation of biphenyl and identified main degradation products. Biphenyl and 2,3-dihydroxybiphenyl (2,3-DHB) was added to cultivation media to identify whole collection of degradation products of four bacterial strains isolated from long-term PCB contaminated soil (Alcaligenes xylosoxidans and Pseudomonas stutzeri) and long-term PCB contaminated sediment (Ochrobactrum anthropi and Pseudomonas veronii). Cultivation flasks were processed in different time after inoculation to determine biphenyl fission rate. Alcaligenes xylosoxidans was revealed as the most appropriate strain for bioremediation process with the highest biphenyl transformation rate. Biphenyl degradation led to the formation of benzoic acid. However, as the presence of 2-hydroxy-6-oxo-6-phenylhex-2,4-dienoic acid (HOPDA) was not confirmed, the transformation pathway common for many other bacteria is probably modified.
Keywords: bacteria, biodegradation, biphenyl, degradation products, 2,3-dihydroxybiphenyl
Full paper in Portable Document Format: acs_0185.pdf
Acta Chimica Slovaca, Vol. 7, No. 1, 2014, pp. 44—51, DOI: 10.2478/acs-2014-0009