Characterization of vine varieties by SSR markers
Ivana Dokupilová *, Ernest Šturdík a, Daniel Mihálik b
Department of Biotechnology, Faculty of Chemical and Food Technology of Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovakia
a Department of Biotechnology, Faculty of Natural Science of University Ss. Cyril and Metodius in Trnava, J. Herdu 2, 917 01 Trnava, Slovakia
b Department of Molecular Biology, The Plant Production Research Centre — Piešťany, Institute of Viticulture and Enology, Bratislavská cesta 122, 92168 Piešťany, Slovakia
E-mail: * dokupilova.ivana@gmail.com
Abstract: Nowadays, there are well known PCR-based techniques to detect polymorphism in plants. Marker technologies which are applied in breeding and varietal characterization can be exchanged across laboratories with standardization to yield reproducible results. This article describes applying of simple sequence repeats (SSR) markers in European laboratories, in which the reproducibility of these popular markers are examined. One of the European Union interests is characterization of grape varieties and preparation of International Vitis catalogue for comparison global varieties as Chardonnay, Sauvignon, Pinot noir with the domestic for each other country. Grape genome contains of many SSR, which are highly polymorphic. This review provides the latest information in SSR research including novels in research, comparison, development and application of microsatellites.
Keywords: Microsatellites — SSR, vine varieties, polymorphism, Vitis vinifera
Full paper in Portable Document Format: acs_0170.pdf
Acta Chimica Slovaca, Vol. 6, No. 2, 2013, pp. 227—234, DOI: 10.2478/acs-2013-0035