Photocytotoxicity of [Cu(N-salicyliden-L-glutamato · 2H2O] · isoquinoline
Jana Pisarčíková, Lýdia Čižeková, Helena Paulíková, Zuzana Vantová * a
Department of Biochemistry and Microbiology, Faculty of Chemical and Food Technology,
Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovak Republic
a Department of Chemical and Biochemical Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovak Republic
E-mail: * zuzana.vantova@gmail.com
Abstract: The metal complexes have a significant role in photodynamic therapy (PDT). The most common metals studied for PDT of tumors are transitional metals. In this paper we have studied the copper(II) complexes of N-salicyliden-L-glutamate. The photocytostatic effect was monitored against mouse leukemic cell line L1210. Photocytostatic effect was confirmed for [Cu(N-salicyliden-L-glutamato·2H2O]·isoquinoline (CuCIQ), but it wasn’t observed for the parental molecule [Cu(N-salicyliden-L-glutamato·2H2O]·H2O. CuCIQ had photocytotoxic effect already at 10 mM concentration. Irradiated cells (1050 mJ.cm–2, l = 365 nm) had half the viability of the cells incubated with CuCIQ in the dark (72 h). Co-incubation of the cells with CuCIQ and ascorbic acid increased this photocytotoxic effect and this effect correlated with the elevated level of lipoperoxidation.
Keywords: photodynamic therapy, photosensitizers, metal complexes, ascorbic acid
Full paper in Portable Document Format: acs_0145.pdf
Acta Chimica Slovaca, Vol. 6, No. 1, 2013, pp. 55—59, DOI: 10.2478/acs-2013-0010